Fig. 1: OligOMM12 colonized mice display a stronger body weight loss but improved viral clearance associated with an enhanced Th1 immune response. | Nature Communications

Fig. 1: OligOMM12 colonized mice display a stronger body weight loss but improved viral clearance associated with an enhanced Th1 immune response.

From: A specific microbial consortium enhances Th1 immunity, improves LCMV viral clearance but aggravates LCMV disease pathology in mice

Fig. 1

SPF (gray), OligOMM12-colonized (OMM12, blue) and germ-free (GF, pink) mice were intravenously infected with LCMV Clone-13 and analyzed at day 8 post infection (dpi). A Illustration of the experimental groups and setup. Created in BioRender (https://BioRender.com/q80p879). B Percentage of body weight loss of indicated groups at 4 and 8 dpi. Dotted line indicates 20% body weight loss (endpoint criteria). P values are as followed: 4 days post infection (SPF vs OMM12 = 0.0052, OMM12 vs GF = 0.0107) and 8 days post infection (SPF vs OMM12 < 0.0001, OMM12 vs GF = 0.0034). C Plots depict plaque forming units (PFU/g) in the small intestine and spleen of GF, SPF and OMM12 colonized animals at 8 dpi. P values are as followed: small intestine (SPF vs OMM12 = 0.0096, OMM12 vs GF = 0.05) and spleen (SPF vs GF = 0.0061 and OMM12 vs GF = 0.0048). D Representative flow cytometry plots of Th1 (T-bethi of CD4+ T cells (E) cell frequencies and absolute cell numbers in small intestine and spleen. P are as followed for small intestine (SPF vs OMM12 = 0.0017 and SPF vs GF = 0.0032), for absolute Th1 counts in small intestine (SPF vs OMM12 = 0.0010 and OMM12 vs GF < 0.0001) and in spleen (SPF vs GF = 0.0161). F Representative flow cytometry plots and frequencies (G) of cytokine+ CD4+ T cells after restimulation with the virus-peptide gp61. P values are as followed: IFNγTNFα small intestine (SPF vs OMM12 = 0.0013 and SPF vs GF = 0.0071), total IFNγ small intestine (SPF vs. OMM12 = 0.0109), spleen (SPF vs OMM12 = 0.0213) and total TNFα small intestine (SPF vs OMM12 = 0.0064 and OMM12 vs GF = 0.0037). For small intestine samples of two animals of the same group were occasionally pooled to increase the yield. Data from two independent experiments are shown. SPF n = 9, OMM12 n = 6, GF n = 8 mice. Each dot represents an individual mouse (or occasionally pooled cells from two individual mice of the same group) and mean ± SD from at least two independent experiments is shown. Statistical analysis for (B, C, E, and G) was performed by using one-way ANOVA with Tukey correction for multiple comparison. P value of <0.05 was considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.

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