Fig. 4: SMARTA cells are transcriptionally different among SPF, OMM12 and GF mice. | Nature Communications

Fig. 4: SMARTA cells are transcriptionally different among SPF, OMM12 and GF mice.

From: A specific microbial consortium enhances Th1 immunity, improves LCMV viral clearance but aggravates LCMV disease pathology in mice

Fig. 4

SPF (gray), OligOMM12-colonized (OMM12, blue) and germ-free (GF, pink) mice received 5000 adoptively transferred SMARTA T cells 5 days prior infection with LCMV Cl-13. SMARTA T cells (live/dead− CD4+CD45.1+) cells from small intestine lamina propria and spleen of 3–5 mice per group were re-isolated at 8 dpi and subjected to RNAseq analysis. A UMAP depicting Louvain cluster for all cells or (B) for each organ and microbiome condition individually. C Relative distribution of clusters in each sample group. Numbers inside the bars indicate the percentage of cells within a specific cluster. D Heatmap of expression levels for the topmost significant cluster-defining transcripts. E Feature plot of the average expression and percentage of cells from each cluster for selected genes.

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