Fig. 2: Myelopoiesis is temporally dynamic in medullary and extramedullary organs in progressive EAE.
From: Myelopoiesis is temporally dynamic and is regulated by lifestyle to modify multiple sclerosis
a Schematic design of experiment. Progressive EAE was induced in female C57BL/6J mice, 9 weeks old, by administering myelin oligodendrocyte glycoprotein (MOG35-55) mixed with complete Freud’s adjuvant (CFA) by subcutaneous injection of day 0 and intraperitoneal injection of PTX on days 0 and 1. Naïve C57BL/6 mice were used as controls. Mean clinical disease scores over the course of 21 days shows monophasic disease. Tissue was collected during the progressive and chronic phases of the model. (n = 16) (b) Quantification of CD45+ leukocyte populations in the spinal cord (n = 5 Naïve, n = 12 progressive, n = 14 chronic). c Quantification of Ly6Chi monocytes and neutrophils in the blood (n = 13 Naïve, n = 14 progressive, n = 10 chronic). d Flow cytometry analysis of bone marrow hematopoietic progenitors. Quantification of LSKs and MPP3 leukocyte progenitors in the femur bone marrow by ELISA (n = 15 Naïve, n = 14 progressive, n = 10 chronic). e Measurement of M-CSF in femur bone marrow (n = 14 Naïve, n = 8 progressive, n = 5 chronic). f Quantification of LSKs and MPP3 leukocyte progenitors in vertebral bone marrow (n = 16 Naïve, n = 14 progressive, n = 9 chronic). g Measurement of splenic weight (n = 11 Naïve, n = 14 progressive, n = 11 chronic) and enumeration of LSKs and MPP3 leukocyte progenitors in the spleen (n = 6 Naïve, n = 8 progressive, n = 6 chronic). One-way ANOVAs. h Circulating blood leukocytes in non-MS population controls (n = 473,905) and MS patients (n = 1918) from the UK Biobank. Data presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are a combination of 2, 3 individual experiments. b-g one-way ANOVA, h two sided Mann-Whitney U test. Created in BioRender. McAlpine, C. (2025) https://BioRender.com/kvggmpp. EAE experimental autoimmune encephalomyelitis, MOG myelin oligodendrocyte glycoprotein, CFA complete Freud’s adjuvant, PTX pertussis toxin, LSK Lin-Sca1+cKit+, MPP3 myeloid-biased multipotent progenitor 3, M-CSF macrophage colony stimulating factor, ELISA enzyme-linked immunosorbent assay, MS multiple sclerosis.
