Fig. 5: M-CSF blockade blunts sleep disruption- and cardiometabolic syndrome-induced myelopoiesis and disease in P-EAE mice.
From: Myelopoiesis is temporally dynamic and is regulated by lifestyle to modify multiple sclerosis
a Experimental schematic of M-CSF blockade with an ɑ-M-CSF antibody in Hcrt−/− or Apoe−/− HFD mice beginning at induction of P-EAE. Mice were injected every other day until symptom onset then daily thereafter. b Ly6Chi monocyte in the blood at pre-onset (day 8) in Hcrt−/− mice with IgG or ɑ-M-CSF (n = 3 IgG, n = 7 ɑ-M-CSF) (c) Myelopoiesis parameters at day 21 in Hcrt−/− mice with IgG or ɑ-M-CSF (n = 9 IgG, n = 7 ɑ-M-CSF). d Clinical scoring in Hcrt−/− mice with IgG or ɑ-M-CSF (n = 10 IgG, n = 7 ɑ-M-CSF). e Ly6Chi monocyte in the blood at pre-onset (day 8) in Apoe−/− HFD mice with IgG or ɑ-M-CSF (n = 3 IgG, n = 6 ɑ-M-CSF). f Myelopoiesis parameters at day 21 in Apoe−/− HFD mice with IgG or ɑ-M-CSF (n = 7 IgG, n = 8 ɑ-M-CSF). g Clinical scoring in Apoe−/− HFD mice with IgG or ɑ-M-CSF (n = 8 IgG, n = 7 ɑ-M-CSF). Data presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are from a representative experiment. b, e two sided Mann Whitney U tests, d, g two-way ANOVA. Created in BioRender. McAlpine, C. (2025) https://BioRender.com/kvggmpp. EAE experimental autoimmune encephalomyelitis, HFD high-fat diet, Apoe−/− Apolipoprotein E knockout, LSK Lin-Sca1+cKit+, MPP3 myeloid-biased multipotent progenitor.
