Fig. 3: SAMD9 binds to dsDNA and dsRNA. | Nature Communications

Fig. 3: SAMD9 binds to dsDNA and dsRNA.

From: SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity

Fig. 3

A Coomassie stain of His-SAMD9 protein analyzed by native-PAGE (left) or SDS-PAGE (right) electrophoresis. B Electrophoretic mobility shift assay (EMSA) of circular plasmid dsDNA with an increasing amount of SAMD9. C Measurement of the dissociation constant (KD) of circular dsDNA with His-SAMD9, calculated based on a half-maximum binding on a curve fit. D EMSA of SAMD9 with linear dsDNA fragments of indicated lengths. E EMSA of His-SAMD9 with 80-bp dsDNA or 80-bp ssDNA. F EMSA of SAMD9 with linear dsRNA fragments of indicated lengths. G EMSA of His-SAMD9 with HMW poly(I:C) or single-stranded RNA polyuridine (ssRNA polyU). H EMSA of His-SAMD9, provided in 2-fold serial dilutions, with 512-bp dsRNA. I KD measurements of 512 bp dsRNA with His-SAMD9, calculated based on a half-maximum binding on a curve fit. For all figures, experiments were repeated at least three times.

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