Fig. 1: Schematic representation of the homing strategy. | Nature Communications

Fig. 1: Schematic representation of the homing strategy.

From: A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Fig. 1

To generate homozygous knock-out ookinetes and oocysts (KO) after pooled transfection, modified PlasmoGEM vectors expressing two sgRNAs separated by hammerhead (HH) and hepatitis delta virus (HDV) ribozymes and Cas9 are brought together in the zygote at the point of fertilisation. gRNA1 and gRNA2 can function independently or in combination to induce a double-stranded break in the parental genome that has an intact allele. The disrupted allele in one parental genome is presumed to serve as a repair template for a Cas9-mediated double-strand break in the other parental genome.

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