Fig. 2: Colour-swapping of tagged MyoA variants as proof-of-principle for homing postfertilisation. | Nature Communications

Fig. 2: Colour-swapping of tagged MyoA variants as proof-of-principle for homing postfertilisation.

From: A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Fig. 2

a Schematics of myoA tagging vectors. The modified parasites carry an expression cassette for MyoA-GFP and two different gRNAs targeting the myoA gene and the myoA 3′ UTR, respectively, for improved cleavage efficiency. In tagged loci, the former target site carries a silent shield mutation, and the latter lacks the PAM site so that tagged loci can no longer be cleaved by Cas9. b Representative microscopic images of mouse blood (input) and oocysts on dissected mosquito midguts 12 days post-infection (output). Mouse blood was stained with Hoechst (blue) before imaging. Oocysts were scored as expressing GFP (green), mCherry (red) or both (yellow). Plots show the relative abundance of oocysts by colour as arithmetic means and standard deviations of 27–54 mosquitoes from two transmission experiments. To obtain accurate homing rates, each mosquito included in the analysis carried at least ten oocysts (mean 501 ± 180 standard deviation).

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