Fig. 3: Pilot screen using CRISPR homing. | Nature Communications

Fig. 3: Pilot screen using CRISPR homing.

From: A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Fig. 3

a Schematic illustration of screen design. A male-only Cas9-expressing line was mutagenised with either homing or standard PlasmoGEM vectors and then crossed with a line providing female gametocytes (also expressing Cas9). Sampling points for barcode counting are indicated. b Oocyst conversion rates were calculated from the relative abundances of barcodes in the output (mosquito midgut, BO) vs. input (mouse blood, BI2) samples. Known stage-specific blocks of clonal mutants are displayed above the graph. c Volcano plot showing how homing affects barcode abundance at the oocyst stage, shown as log2 fold change in the presence of gRNA (+homing) compared to the no-gRNA control (-homing). Each circle represents a gene. Effect sizes are plotted against the −log10 p-values calculated using a Z-test. The dotted line shows a p-value of 0.05. Mutants with male fertility were lost from the pools irrespective of homing and were, therefore, omitted from this analysis. Data are from biological triplicates from a total of at least 100 mosquitoes per condition.

Back to article page