Fig. 4: Immunofluorescence micrographs localising CRTL-3xHA. | Nature Communications

Fig. 4: Immunofluorescence micrographs localising CRTL-3xHA.

From: A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Fig. 4

Midguts infected with wild-type parasites and parasites carrying a 3xHA tag on CRTL were dissected and fixed on different days post-infection. Immunostaining against HA is shown in green, and Hoechst-stained DNA is shown in blue. The schematic above illustrates the digestive vacuole-like structures (DV-like), nuclei (N), sporoblast areas (SB) and sporozoites (SP) at different stages of oocyst development. The data represent two separate transmission experiments, each involving the analysis of 25 infected mosquito midguts at the indicated time point.

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