Fig. 5: Characterisation of a cloned CRTL mutant. | Nature Communications

Fig. 5: Characterisation of a cloned CRTL mutant.

From: A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Fig. 5

a Oocyst numbers on 25 dissected guts from infections with Pb Bergreen (WT, green) or Pb mCherry crtl− (red) parasites (n = 25). The data are representative of two transmission experiments. (ns not significant in unpaired T-test). b Growth kinetics of oocysts from 30 infected mosquitoes per time points. Oocyst diameter from randomly selected midguts was measured using ImageJ and plotted (n = 125 ± 50). (ns not significant; ***P < 0.001; ****P < 0.0001 in unpaired T-test) c Representative images illustrating oocyst size differences on a day 10 oocyst. d Representative images of day 8 oocysts highlighting acidic compartments with Lysotracker staining. The boxed area is enlarged on the right to show a DV-like acidic compartment in a WT oocyst. V vacuolar structures that appear in crtl− oocysts. e Representative transmission electron (TEM) micrographs of day 10 oocysts. Boxed areas are enlarged to show sporoblast (Sb), capsule (Cap) and sporozoite (Sp) in WT oocyst. crtl− oocysts are smaller, lack sporoblast and have enlarged vacuolar structures (V) despite having an intact capsule (cap). The data is from 2 independent replicates. f Representative images of a set of salivary glands of WT or crtl− infected mosquitoes 20 days post-infection.

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