Fig. 1: Development of iPE for inverse prime editing in human cells.

a Schematic diagram of the canonical prime editor (PE) using nCas9-H840A. The red region of the genome represents the DNA segment that can be edited by PE. b Schematic diagram of the inverse prime editor (iPE) using nCas9-D10A. The red region of the genome represents the DNA segment that can be edited by iPE. c Inverse prime editing frequencies achieved by four different iPE constructs, including iPE2–5, at target sites HBB, HEXA, FANCF, and PDCD1 in HEK293T cells. d Inverse prime editing frequencies achieved by eight different iPE constructs, including iPE2–5 and iPEmax2–5, at target sites DMD, ETS1-T1, FANCF, and PAH in HEK293T cells. Frequencies (mean ± s.e.m.) in c-d were obtained from three biological replicates (n = 3). Ins, insertion; Del, deletion. InDels, byproducts of random insertions and deletions. Source data are provided as a Source Data file.