Fig. 5: Off-target effects of circular RNA-mediated inverse prime editors.
From: Circular RNA-mediated inverse prime editing in human cells
a Mutation frequencies achieved using ciPEs at three on-target and 26 off-target sites identified by Cas-OFFinder for GFAP, HEK4, and DMD in HEK293T cells. b Mutation frequencies achieved using Rep-X-assisted ciPEs at three on-target and 26 off-target sites identified by Cas-OFFinder for GFAP, HEK4, and DMD in HEK293T cells. Frequencies (mean ± s.e.m.) in a, b were calculated from three biological replicates (n = 3). The sequences in the red box represent similar primer binding sequences downstream of the cleavage site between on-target and off-target at the HEK4 target site. Del, deletion. InDels, byproducts of random insertions and deletions. Source data are provided as a Source Data file.
