Fig. 4: ALF can be used to predict PA intensity.
a Structures of new NR dyes used to investigate the ability of acoustic loudness factor (ALF) to predict changes in PA signal derived from different mechanisms of modulating knr. b Dyes from panel a plotted on the trend line from Fig. 2d. c Photophysical properties and predicted PA intensities from dyes shown in panel a. Photophysical properties were measured in triplicate in DPBS:MeCN (7:3, 1% DMSO). PA emission was measured from quantification of PA images of 1.2 cm thick tissue phantoms at positions 2 mm apart (n > 5). aDichloromethane used as a solvent. bNon-radiative decay rates are calculated from the ALF trend line in Fig. 2d (Supplementary Note 2).
