Fig. 2: Mosaic clones recruit hemocytes.
From: Epithelial cell competition is promoted by signaling from immune cells
Wing imaginal discs carrying no (A), control (B) and dMyc clones (C), 72 h ACI; hemocytes visualized using hml-Gal4 to drive UAS-RFP. (A’-C’) Discs in (A-C) were stained with DAPI. D Quantification of the number of hemocytes in discs of the genotypes in (A), or (B, C) collected at defined time periods--16, 24, 48 or 72 h ACI. n = 11, 15, 9, 15, 9, 10, 8, 12, and 15 discs, respectively. E Comparison between expected and observed distributions of hemocytes with respect to distance from clone boundaries in wing discs of the genotype in C, collected at 48 or 72 h ACI (See Methods). Wild-type (F) and dMyc-overexpressing (G) clones were induced in wing discs, and hemocytes were detected using hml-Gal4 driving UAS-RFP, 72 h ACI. F’ and G’ are higher magnification views of the dashed areas in F and G, respectively. H Numbers of hemocytes in wing discs of the genotypes in F and G; n = 16 and 15 discs, respectively. I Areas of hemocyte nuclei in discs of the genotypes in (F) and (G). n = 24 and 215 hemocytes for the genotypes in (F) and (G), respectively. Observations were subdivided into groups based on the part of the disc examined (Columnar membrane, CM; Peripodial membrane, PM; Edge). *P < 0.05; **P < 0.01; ***P < 0.001 by two-sided Mann-Whitney U test. P value = 8.95 × 10−4, 1.23 × 10−2, 4.27 × 10−3, 1.86 × 10−2, 3.44 × 10−3, and 3.42 × 10−4, respectively. Bar = 50 µm. In each box and whisker plot, the box represents the interquartile, spanning from the first quartile to the third quartile, with a white line inside marking the median, and the whiskers show the range of the data, reaching the minimum and maximum values. Source data are provided as a Source Data file.
