Fig. 8: Conformational landscape analysis of the main motions detected by HetSIREN on the SARS-CoV-2 Spike protein at 37°C. | Nature Communications

Fig. 8: Conformational landscape analysis of the main motions detected by HetSIREN on the SARS-CoV-2 Spike protein at 37°C.

From: Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN

Fig. 8

Panel a) shows the UMAP19 representation of the landscape obtained from the original 8D latent space encoded by HetSIREN from the particle images after training. Each dot in the landscape corresponds to the position of the cluster representative obtained from a KMeans clustering of the original HetSIREN latent space. Panel b) shows side views of the decoded HetSIREN volumes obtained from the latent space coefficients assigned to every representative shown in Panel a). The maps provide a sensible exploration of the different states identified by HetSIREN, including 3 Down and 1 Up conformations.

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