Fig. 3: Diabetic glomeruli have an imbalance in redox ratio and lipid saturation. | Nature Communications

Fig. 3: Diabetic glomeruli have an imbalance in redox ratio and lipid saturation.

From: Label-free multimodal optical biopsy reveals biomolecular and morphological features of diabetic kidney tissue in 2D and 3D

Fig. 3

a Core biopsies from diabetic patient along the cortical-medullary axis shows consistencies and differences between the optical redox ratio and degree of lipid saturation. Intensity profiles are plotted along this axis by ignoring background pixels, and normalized by setting the areas under each curve equal to each other. b Glomerular subregion images using SRS and TPF multimodal imaging, along with simple SRH processing. Note the mesangiolysis, marked by red blood cell stasis and regions of irregular matrix texture, in the diabetic glomerulus. c Normalized optical redox ratio as a measure of oxidative stress and metabolism in healthy and diabetic glomeruli. d Quantitative image analysis of ORR in glomeruli, n = 9 (3 each from 3 separate biopsies). Two tailed t test ****p = 0.000087446. e Ratiometric images of lipid saturation in healthy and diabetic glomeruli. f Quantitative image analysis of lipid saturation in glomeruli, n = 9 (3 each from 3 separate biopsies). Scale bar, 100 µm. Data presented are interquartile range box plots with median line and 1.5 IQR whiskers. Two tailed t test **p = 0.0075. ORR Optical Redox Ratio, AUC Area Under Curve, SRH Stimulated Raman Histology, NAD[P]H Nicotinamide Adenine [Phosphorylated] Dinucleotide.

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