Fig. 6: Spatial collagen analysis of human kidney biopsies using SHG imaging. | Nature Communications

Fig. 6: Spatial collagen analysis of human kidney biopsies using SHG imaging.

From: Label-free multimodal optical biopsy reveals biomolecular and morphological features of diabetic kidney tissue in 2D and 3D

Fig. 6

a Spatially-mapped fiber thickness of representative SHG images of control and diabetic biopsies along the cortical-medullary axis. Both samples have distinguishably thicker collagen fibers towards the medulla side, as well as peaks of fiber thicknesses in the cortex near glomeruli (marked with green arrows), vessels, and arteries. b The mean fiber thickness of all fibers is displayed overlayed by the corresponding violin outlines of 5000 randomly sampled fiber diameters for each group, as well as the mean SHG intensity for both control and diabetic samples show significantly higher levels in diabetic samples. n = 4 tissue slides per group. c 2D contour plots show the density of collagen fiber thickness measurements versus distance from the nearest glomerulus center in control and diabetic samples (n = 20 glomeruli per group). Representative SHG images of glomeruli are shown to illustrate how fiber thickness tends to diminish further away from the glomerulus. Error bars represent one standard error of the mean. Data presented are mean ± SEM. Two tailed t test *p = 0.0144. SHG Second Harmonic Generation.

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