Fig. 1: Single-nucleus multiomics of E14.5 mouse testes. | Nature Communications

Fig. 1: Single-nucleus multiomics of E14.5 mouse testes.

From: NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development

Fig. 1

A UMAP projection of the single-nucleus multiomics (combined RNA-seq and ATAC-seq) data from E14.5 testes, color-coded by cell clusters. B Dot plot representation of the expression of cell specific marker genes for each cluster. C Feature plots of the normalized expression of interstitial genes on the multiomic UMAP visualization of E14.5 testes. D Immunofluorescence for cluster/cell type specific markers (Yellow) in E14.5 male testes. Samples were counterstained with DAPI (Gray). White arrowheads indicate immunopositive cells. White dashed box indicates the magnified area. E UMAP of the single-nucleus ATAC-seq data from E14.5 testes, color-coded based on the multiomic (combined RNA-seq and ATAC-seq) cell clusters. F Volcano plot of differentially expressed genes between c0 (steroidogenic progenitor cells) and c3 (fetal Leydig cells). Blue and red dots represent significantly upregulated genes in c0 or c3 respectively.

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