Fig. 3: Lineage tracing of Nr2f2-positive cells in the mouse fetal testis. | Nature Communications

Fig. 3: Lineage tracing of Nr2f2-positive cells in the mouse fetal testis.

From: NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development

Fig. 3

A Schematic representation of the lineage tracing experiment of the fetal testis-derived Nr2f2+ cells in the Nr2f2-CreER; CAG-Sun1/sfGFP embryos. Created in BioRender, Yao, H. (2025) https://BioRender.com/ygb4q06. Cre activity was induced by tamoxifen administration at E11.5 (B), E12.5 (C), E13.5 (D) or E14.5 (E). Testes were collected at E14.5 (B–D) or E17.5 (E) and analyzed with immunofluorescence for peri-nuclear Sun1-GFP (green) and NR2F2, NR5A1, HSD3B1 or AMH (magenta). White arrowheads indicate NR2F2-positive cells labeled with Sun1-GFP. Yellow arrowheads indicate NR2F2-negative, Sun1-GFP-positive or Sun1-GFP/NR5A1 or Sun1-GFP/HSD3B1 double positive fetal Leydig cells. White dashed box indicates the magnified area.

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