Fig. 4: Phenotypes of Nr2f2 conditional knockout testes at E14.5. | Nature Communications

Fig. 4: Phenotypes of Nr2f2 conditional knockout testes at E14.5.

From: NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development

Fig. 4

A Principal component analysis (PCA) plot of the bulk RNA-seq of control and Nr2f2 cKO testes. B Volcano plot of differentially expressed genes between the Nr2f2 cKO and the control testes. Blue and red dots represent significantly (adjusted p < 0.05) downregulated and upregulated genes, respectively, in the cKO testis in comparison to the control. C Immunofluorescence for NR2F2, LacZ and different cell type markers (PDGFRA for interstitial cells, ARX for interstitial cells, PECAM1 for endothelial cells, TRA98 for germ cells, AMH for Sertoli cell, HSD3B1 for fetal Leydig cells, and ACTA2 for contractile cells) on E14.5 control or Nr2f2 cKO testes. White dashed box indicates the magnified area. Red arrowheads indicate NR2F2 positive/LacZ negative cells. Blue arrowheads indicate NR2F2 negative/LacZ positive cells. White arrowheads indicate NR2F2 and LacZ double positive cells.

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