Fig. 5: Differences of sex development phenotypes in Nr2f2 cKO male embryos. | Nature Communications

Fig. 5: Differences of sex development phenotypes in Nr2f2 cKO male embryos.

From: NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development

Fig. 5

A Wholemount darkfield images of E17.5 urogonadal system of control and Nr2f2 cKO male embryos. Adrenal glands (Ad), kidneys (Ki), testes (Te) and blader (Bl) are indicated with white arrows. B Immunofluorescence of E17.5 control or Nr2f2 cKO testes for AMH (magenta), HSD3B1 (yellow) and DAPI (cyan). White dashed box indicates the magnified area. C Quantification of the number of fetal Leydig cells per area in control and Nr2f2 cKO testes. Bars represent mean ± s.e.m., n = 6 control and n = 4 Nr2f2 cKO, p = 0.0175. D Percentage of fetal Leydig cells per testis in control and Nr2f2 cKO testes, relative to the control. Bars represent mean ± s.e.m., n = 6 control and n = 5 Nr2f2 cKO, p = 0.0031. Unpaired two-tailed t-test. *P < 0.05; **P < 0.01. E Wholemount darkfield images of E17.5 control and Nr2f2 cKO male genitalia. Black arrowhead represents the positioning of the urethra exit. Black arrow indicates abnormal urethra opening at the base of the genitalia. F Hematoxylin and eosin staining of E17.5 control and Nr2f2 cKO external genital sections at the base of the genitalia. G Immunofluorescence for MAFB (yellow) in E17.5 control and Nr2f2 cKO genitalia section. White dashed line indicates the urethra epithelium (UE).

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