Fig. 6: NR2F2 binding and chromatin accessibility change during interstitial to Leydig cell differentiation.

A Pie chart diagram classifying the significant (fdr < 1⁻⁵) NR2F2 peaks detected based on their location in the genome. B Top 3 known Jaspar binding motif analysis based on the significant (fdr < 1⁻⁵) NR2F2 peaks. C Top 3 de novo Jaspar binding motif analysis based on the significant (fdr < 1⁻⁵) NR2F2 peaks. D Venn diagram contrasting all genes with a significant NR2F2 peaks (cyan) with the differentially expressed genes (DEGs) from steroidogenic progenitor cells (c0, yellow) and fetal Leydig cells (c3, magenta) based on the single-nucleus RNA-seq. E Volcano plot of all the differentially accessible peaks between c0 and c3 (adjusted p < 0.05), color-coded based on the peak to gene linkage information. Expression levels and motif enrichment of the top 5 genes based on the combination of differential expression (adjusted p < 0.05) and the motif enrichment (adjusted p < 0.05) for c0 (F) and c3 (G). H Expression levels and motif enrichment of the top 5 genes based on the combination of differential expression (adjusted p < 0.05) and the motif enrichment (adjusted p < 0.05) for peaks containing significant (fdr < 1⁻⁵) Nr2f2 ChIP-seq peaks in c0.