Fig. 1: Overview of the history of bacterial epigenetics, benchmarking strategy, and Nanopore sequencing data generated in this study.
From: Comprehensive comparison of the third-generation sequencing tools for bacterial 6mA profiling
a Timeline of important events in bacterial DNA modification studies throughout the past century. Key milestones are highlighted, with two major techniques of the TGS colored blue and orange. Other types of 6mA detection technologies are listed within the green dashed line box, while the orange dashed line box marks the five tools (Tombo has three sub-tools) for 6mA detection on Nanopore data that are evaluated in this research. b Diagram describing the processing workflow and benchmarking strategy. The left dashed line separates PacBio tools and ONT tools. The right dashed line separates the two types of sequencing files and corresponding tools from the flow cell R9.4.1 and R10.4.1. Created in BioRender. Huang, J. (2025) https://BioRender.com/x63f659. c Quality statistics of sequencing outputs based on four features. The gray color represents unmapped reads or bases. Centre line: median. Box bounds: 25th to 75th percentiles (interquartile range, IQR). Whiskers: extend to most extreme data points within 1.5 × IQR. WT wild type, WGA whole genome amplification. The n number for coverage is the genome size of Psph (n = 6538260).
