Fig. 3: Mitochondrial dynamics is stimulated by nitro-oleic acid in LV of mice with HFpEF. | Nature Communications

Fig. 3: Mitochondrial dynamics is stimulated by nitro-oleic acid in LV of mice with HFpEF.

From: Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in mice

Fig. 3

Identification of mitochondrial proteins (a) and localization (b) by mitoCarta29. a Heatmap of mitochondrial proteins detected by liquid chromatography–mass spectrometry in left ventricular tissue of mice after chow diet, 15 weeks of high-fat diet (HFD) and L-NAME with 4 weeks of vehicle (HFD + L-NAME vehicle) or NO2-OA treatment (HFD + L-NAME NO2-OA). Protein names and z-scores are in the source data file. b Shown is the percentage of identified mitochondrial proteins (grey) and the percentage of mitochondrial proteins with significantly enhanced abundance after administration of NO2-OA in HFD + L-NAME mice compared to vehicle-treated HFD + L-NAME mice (red). c Protein levels of highly abundant mitochondrial proteins (-log10(p-value) > 4.5; log2(fold change) > 1.9) (N = 9/8/6), ABCD3: ATP-binding cassette subfamily D member 3; COX: cytochrome c oxidase; TOM70: translocase of outer mitochondrial membrane 70; TM177: transmembrane protein 177. d Representative immunoblots (IB) and quantification of COX4 and TOM70 (N = 9/8/6). Protein levels were normalized to the total protein amount assessed on IB by fluorescence labeling (Figs. S12 and S13). e Representative TEM images (N = 5 animals per group) at magnifications of 5 K (scale bar 5 µm), 10 K (scale bar 2 µm), and 31.5 K (scale bar 1 µm), sc: sarcomere; ld: lipid droplet; mt: mitochondria with intact cristae; white arrow: early cristae fragmentation; white asterisk: cristae adhesion; black asterisk: swollen, ruptured mitochondria. f Number of mitochondria normalized by mitochondrial area (24 images per group, N = 4/5/4). g Correlation of f with isovolumetric relaxation time (IVRT). h Relative mitochondrial DNA (mtDNA) copy number and protein abundance of the mitochondrial transcription factor (TFAM). Data are mean ± standard deviation for c, d, f and h. Data in c, d and h are shown relative to the mean of the chow group. Statistical significance was calculated with One-way ANOVA followed by Bonferroni´s post-hoc test for c, d, f and h. Kruskal-Wallis-test followed by Dunn´s multiple comparison test was used for COX1 in c and TOM70 in d. Pearson correlation was used for g. Only statistically significant differences are indicated. p: *<0.05, **<0.01, ***<0.001, ****<0.0001.

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