Fig. 6: Nitro-oleic acid enhances cardiac fatty acid metabolism in HFpEF mice. | Nature Communications

Fig. 6: Nitro-oleic acid enhances cardiac fatty acid metabolism in HFpEF mice.

From: Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in mice

Fig. 6

a Heatmap of all proteins associated with fatty acid metabolism and identified by liquid chromatography mass spectrometry (LC-MS) after administration of NO2-OA in high-fat diet (HFD) + L-NAME treated mouse hearts compared to vehicle-treated mouse hearts. Protein names and z-scores are in the source data file. b Protein abundances of carnitine palmitoyltransferase 1B (CPT1B) relative to cytochrome c oxidase 4 (COX4) and long chain fatty acid transporter (FAT/CD36) derived from LC-MS. Data are shown relative to the mean of the chow group (N = 9/8/6). c Representative images of adult murine cardiomyocytes stained for neutral lipids (left). Quantification of lipid droplet area normalized to cardiomyocyte area shown for all individual analyzed images (right) (9 to 10 images at a magnification of 63x per animal, N = 6/5/6). Data are presented as mean ± standard deviation. Statistical significance was calculated by One-way ANOVA followed by Bonferroni´s post-hoc test. Only statistically significant differences are indicated. p: *<0.05, **<0.01, ***<0.001, ****<0.0001.

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