Fig. 1: Different co-TF (Pho2) dependence between orthologous TF (Pho4) in yeast species. | Nature Communications

Fig. 1: Different co-TF (Pho2) dependence between orthologous TF (Pho4) in yeast species.

From: Divergence in a eukaryotic transcription factor’s co-TF dependence involves multiple intrinsically disordered regions

Fig. 1

A Cartoon summary of previous work: S. cerevisiae Pho4 (ScPho4) depends on the co-TF, Pho2, for DNA binding and activation of Phosphate Starvation (PHO) Response genes (top row), while Pho4 from C. glabrata (CgPho4) can bind and activate without the co-TF. See text for references. B Two non-mutually exclusive models to explain the divergence in Pho2-dependence. C The tiles in the middle show the conservation scores at aligned positions of the two Pho4 orthologs; the box diagrams represent ScPho4 and CgPho4, with gray lines connecting matching residues to the alignment tiles. Five regions were delineated based on knowledge about ScPho4: R1 = regulatory, interact with Pho80; AD activation domain, NLS nuclear import and export signals, P2ID Pho2-interaction domain (also includes a region contacting Pho80), DBD basic Helix-Loop-Helix DNA binding domain. The lines and cylinders above and below the region diagrams show predicted α helices in each protein, with lighter color corresponding to lower prediction confidence. Prediction was done using PSIPRED 4.0.

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