Fig. 3: CgPho4 encodes two Activation Enhancer Domains (AEDs) that can enhance the activation potential of the main AD. | Nature Communications

Fig. 3: CgPho4 encodes two Activation Enhancer Domains (AEDs) that can enhance the activation potential of the main AD.

From: Divergence in a eukaryotic transcription factor’s co-TF dependence involves multiple intrinsically disordered regions

Fig. 3

A, B PADDLE prediction of activation potential in ScPho4 and CgPho4. Predictions were made in 53 aa blocks and plotted using the middle of each block as the x coordinate. As a result, the first and last 26 aa positions have no scores. Z-scores > 4 and 6 indicate regions with medium and strong activation potential, respectively. The five regions in each protein are labeled as in Fig. 1. Orange triangles mark the 9aaTAD motif matches. Below the plots are diagrams of the Gal4DBD fusion constructs used to test the activation potential, with their boundaries labeled on the right. C Activation potential of each region was tested in the context of a Gal4 DBD fusion driving a GAL1pr-mCherry reporter. Shown are the mean (bar) and individual data points (n = 3, except for the host, for which n = 6, biological replicates). Host has the mCherry reporter but no plasmid; Gal4_DBD has the reporter and the Gal4 DBD alone. A linear model with construct as the independent variable was used to compare the Gal4 DBD fusions to the two negative controls combined; an asterisk indicates significant difference at a 0.05 level after Holm-Bonferroni correction. D CgE1 and CgE2_9aa can enhance the activation capability of ScAD_9aa in an orientation-independent manner. Plot design and replicate numbers are the same as in (C). An asterisk indicates significance difference at a 0.05 level by an unpaired, two-sided Student’s t-test between the indicated groups after Holm-Bonferroni correction.

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