Fig. 7: Summary of Pho4 protein divergence and its contribution to the Pho2-dependence variation in ScPho4 and CgPho4. | Nature Communications

Fig. 7: Summary of Pho4 protein divergence and its contribution to the Pho2-dependence variation in ScPho4 and CgPho4.

From: Divergence in a eukaryotic transcription factor’s co-TF dependence involves multiple intrinsically disordered regions

Fig. 7

Left (1): CgPho4 DBD recognizes the same DNA sequence as ScPho4 DBD, but binds the consensus motif with a 3–4 fold higher affinity. While consistent with the enhanced activity model, further studies found no evidence for the enhanced activity to be a major factor for the reduced Pho2-dependence. (2) Two regions in the IDR of CgPho4 (red asterisks) can enhance the activity of the main activation domain (AD) but are not sufficient on their own to activate gene expression. These two Activation Enhancing Domains (AEDs) directly contribute to CgPho4’s reduced Pho2-dependence. Right (3): An IDR (green asterisk) adjacent to the DBD in ScPho4, named P2ID, can both interact with ScPho2 and boost the activity of Pho4 and also repress its activity when Pho2 is absent, providing support for the autoinhibition model.

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