Table 1 Breakpoints for chimeric Pho4 and individual regions tested in this study

Origin	Region Name	Start	End	Length	Used in figure(s)
CgPho4	R1	1	44	44	Figs. 1, 4, 5, 6, S2, S4
CgPho4	AD	45	112	68	Figs. 1, 4, 5, 6, S2, S4
CgPho4	NLS	113	282	170	Figs. 1, 4, 5, 6, S2, S4
CgPho4	P2ID	283	458	176	Figs. 1, 4, 5, 6, S2, S4
CgPho4	DBD	459	533	75	Figs. 1, 4, 5, 6, S2, S4
ScPho4	R1	1	42	42	Figs. 1, 4, 5, 6, S2, S4
ScPho4	AD	43	99	57	Figs. 1, 4, 5, 6, S2, S4
ScPho4	NLS	100	176	77	Figs. 1, 4, 5, 6, S2, S4
ScPho4	P2ID	177	242	66	Figs. 1, 4, 5, 6, S2, S4
ScPho4	DBD	243	312	70	Figs. 1, 4, 5, 6, S2, S4
CgPho4	P2ID first half	283	327	45	Figs. 6, S2
CgPho4	P2ID second half	328	458	131	Figs. 6, S2
ScPho4	P2ID first half	177	204	28	Figs. 6, S2
ScPho4	P2ID second half	205	242	38	Figs. 6, S2
CgPho4	E1	2	44	43	Figs. 3, S5
CgPho4	AD	45	126	82	Figs. 3, S5
CgPho4	E2	252	463	212	Figs. 3, S5
CgPho4	AD.1	45	75	31	Figs. 3, S5
CgPho4	AD.2	76	126	51	Figs. 3, S5
CgPho4	E2_9aa	252	288	37	Figs. 3, S5
ScPho4	E1	2	42	41	Figs. 3, S5
ScPho4	AD	43	101	59	Figs. 3, S5
ScPho4	AD_9aa	70	101	32	Figs. 3, S5
ScPho4	E2	156	250	95	Figs. 3, S5
ScPho2	AD	404	559	156	Fig. 3
ScPho4	Alternative NLS	100	156	57	Fig. 5
CgPho4	Alternative P2ID	253	463	211	Fig. 5
ScPho4	ΔbHLH	2	250	249	Fig. 6 Y2H
CgPho4	ΔbHLH	2	463	462	Fig.6 Y2H
ScPho2	Pho4int	112	407	296	Fig. 6 Y2H
CgPho4	purified DBD	452	533	82	Figs. 2, S4
ScPho4	purified DBD	236	312	77	Figs. 2, S4

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