Fig. 1: Individual nodose ganglia sensory neurons respond in a cytokine‐specific manner.
From: Neural representation of cytokines by vagal sensory neurons
a PHOX2B and PRDM12 labeling reveals distinct subsets of placode‐derived and neural crest‐derived vagal sensory neurons in the jugular‐nodose ganglionic complex. Scale bar, 100 µm. IHC was repeated independently 5 times. b Schematic of Miniscope in vivo calcium imaging of the mouse vagal ganglia. Top images show (left to right): raw fluorescence signal, DFF map, and a region-of-interest map showing active neurons; scale bar, 50 µm. Plot shows example individual calcium transient traces from six active neurons. c Distinct neuronal responses to specific cytokines (IL‐1β, 200 ng/mL; TNF, 50 ng/mL, and IL‐10, 50 ng/mL) administered to the vagus nerve. d Sensory neuron responses to cytokines are different from one another in their DFF amplitude (mean ± SEM, per mouse, n = 9, 10, 11, left to right, IL-1β vs TNF, *** P = 0.0023, IL-1β vs IL-10 and TNF vs IL-10, **** P < 0.0001, two-sided, one-way ANOVA, Tukey test corrected for multiple comparisons).
