Fig. 3: Differential gene expression analysis comparing concurrent cerebral malaria and severe malarial anemia to uncomplicated controls without a history of cerebral malaria. | Nature Communications

Fig. 3: Differential gene expression analysis comparing concurrent cerebral malaria and severe malarial anemia to uncomplicated controls without a history of cerebral malaria.

From: A shared inflammatory signature across severe malaria syndromes manifested by transcriptomic, proteomic and metabolomic analyses

Fig. 3

A Principal component analysis showing the separation of concurrent CM and SMA from UM along the first two principal components. B Volcano plot showing the significance of association from a quasi-likelihood F test based on the negative binomial distribution (red FDR-adjusted p-value < 0.05) and effect size (green and red >1 absolute log2-fold change). C Heatmap of the top 20 differentially expressed transcripts, showing the clustering of associated genes from a quasi-likelihood F test (y-axis) and CM cases versus UM controls (x-axis). D Bar plot of the top 10 significantly associated gene ontology biological processes. Enrichment analysis was performed using the clusterProfiler package in R. Enriched terms were determined using a hypergeometric test, with p-values < 0.05 considered significant. Pathway names are represented on the Y-axis, and gene counts on the X-axis. Color spectrum represents the extent of statistical association, from red, p-value 9E-06, to blue, p-value 3E-05.

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