Fig. 6: Bile acid components and pro-inflammatory cytokines dampen the expansion and/or CXCR6 expression of MAIT cells.

A Spearman’s correlation between the serum bile acids levels with frequencies of cMAIT (n = 68) and hMAIT (n = 72). Data were collected from at least ten independent experiments and are presented as mean ± SEM. B Correlation heatmap depict the relationships between hMAIT frequencies with hepatic bile acids levels and transcript levels of pro-inflammatory cytokines in liver tissue from hMAIT-LF (n = 5), hMAIT-HBVN (n = 4), and Comp (n = 5), with colors showing correlation index levels. C Representative dot plots of the frequencies expanded MAIT cells in the presence of glycochenodeoxycholic acid (GCDCA), glycoursodeoxycholic acid (GUDCA), taurochenodesoxycholic acid (TCDCA), IL-1β, IL-6, IL-7, IL-8. D The summarized graph of the frequencies and numbers of expanded MAIT cells in the blank and aAPCs with medium, DMSO (n = 8), GCDCA, GUDCA, TCDCA (n = 8/group). Data were collected from eight independent experiments and are presented as mean ± SEM. One color in different groups represents the same donor. Statistical significance was assessed by one-way ANOVA multiple comparisons. E The summarized graph of the frequencies expanded MAIT cells in the blank (n = 10) and aAPCs with medium (n = 10), IL-1β (n = 8), IL-6 (n = 8), IL-7 (n = 8), IL-8 (n = 4). Data were collected from eight independent experiments and are presented as mean ± SEM. One color in different groups represents the same donor. Statistical significance was assessed by one-way ANOVA multiple comparisons. F Representative and summarized CXCR6 levels on MAIT cells in the presence/absence of indicated bile acids or cytokines (n = 4/group). Data were collected from four independent experiments. Data are presented as mean ± SEM. n-values represent biological replicates. Statistical significance was assessed by one-way ANOVA multiple comparisons. Source data are provided as a Source Data file.