Fig. 1: Impaired homeostatic expansion of Ant2^−/− T cells.

A Representative flow cytometry plots showing the frequency of CD4⁺ and CD8⁺ T cells in the spleen from WT and Ant2^−/− littermate mice (6–9 weeks old). B Bar graphs summarizing the results in (A), indicating percentages (left) and absolute cell numbers (right) (n = 6). C–F Analysis of thymus development of WT and Ant2^−/− littermate mice (6–9 weeks old). C Left panel: representative flow cytometry plots showing four developmental stages (A–D), distinguished by CD69 vs TCRβ staining of whole thymocytes. Right panel: CD4 vs. CD8 staining gated on each of the developmental stages, (A–D). D Bar graph summarizing the results shown in (C), left panel (n = 5). E Representative flow cytometry plots showing the frequency of CD4 vs. CD8 in the thymus-derived from 6 to 9 weeks old WT and Ant2^−/− littermate mice. F Bar graph summarizing the results of panel (F). DP-double positive, DN-double negative, and SP-single positive. G Schematic of homeostatic expansion experiment; co-adoptive transfer of CFSE-labeled WT, and CellTrace-labeled Ant2^−/− -derived splenocytes to sub-lethally irradiated recipient mice. The reciprocal experiment was also performed. H Representative flow cytometry plots showing CFSE vs CellTrace intensities gated on donor CD8⁺ CD62L^hi T cells. I Graph depicting paired proliferation indexes of donor WT and Ant2^−/− CD8⁺ T cells (n = 8). J–K Ki-67 and cell cycle analysis of CD8⁺ T cells (J) Representative flow cytometry plots of DAPI vs Ki-67 gated on CD8⁺ T cells. K Bar graphs summarizing the results in (J), as the percentages of cells in each of the cell cycle stages (left) and total gMFI of Ki-67 (right) (n = 5). Statistical method: Two-tailed Mann–Whitney test (B, D, F, K), or two-tailed Wilcoxon matched-pairs signed rank test (I). Data are represented as mean ± S.D. n refers to the number of biologically independent samples. ns = not significant. Source data are provided as a Source Data file.