Fig. 2: Intact T-cell mediated immunity in Ant2^−/− mice.

A Schematic representation of the immunization experiment used to estimate CD4⁺ T-cell responses. WT and Ant2^-/- mice were immunized three times at 7-day intervals. The first two immunizations included OVA protein in complete Freund’s adjuvant (CFA), while the third included OVA without CFA. One week after the third injection the concentration of, OVA-specific IgG1 (WT; n = 10, Ant2^−/−; n = 9) (B) and IgG2a (WT; n = 11, Ant2^−/−; n = 8) (C), as well as the cytokines IFNγ (WT; n = 12, Ant2^-/-; n = 9) (D) and IL-4 (WT; n = 14, Ant2^−/−; n = 7) (E), were measured in the serum of the mice using enzyme-linked immunosorbent assay (ELISA). F–K WT and Ant2^−/− littermate mice were primed intradermally in the left ear-pinna with 5 × 10⁶ transduction units (TU) of lentivirus expressing OVA (LvOVA). Seven days post-infection, cervical lymph nodes (LN) were dissected, and single cells were stained for activation-related surface markers. F Representative flow cytometry plots showing CD62L vs. CD44 gated on CD8⁺Vα2⁺ T cells. G Bar graph summarizing the results shown in F (WT; n = 6, Ant2^−/−; n = 5). H Absolute cell number of CD8⁺Vα2⁺CD44⁺CD62L⁻ in the cervical LN of WT and Ant2^−/− mice. I Representative flow cytometry plots showing CD62L vs. CD25 gated on CD8⁺Vα2⁺ T cells. (J): Bar graph summarizing the results shown in (I) (WT; n = 6, Ant2^−/−; n = 5). K Absolute cell number of CD8⁺Vα2⁺CD25⁺CD62L⁻ in the cervical LN of WT and Ant2^−/− mice. Statistical method: Two-tailed Mann–Whitney test. Data are represented as mean ± S.D. n refers to the number of biologically independent samples. ns =  not significant. Source data are provided as a Source Data file.