Fig. 4: Phylogenetic analysis of avian influenza virus (AIV) neuraminidase (NA) gene sequences obtained from bird guano samples, alongside reference sequences from GISAID.

Panels show phylogenies for the following NA subtypes: a N1, b N2, c N3, d N4, e N5, f N7, g N6, and h N9. Evolutionary history was inferred using the Maximum Likelihood method and the Hasegawa-Kishino-Yano substitution model for the hemagglutinin (HA) and neuraminidase (NA) genes. The trees with the highest log likelihood are shown. Nextclade (https://clades.nextstrain.org) were used for clade assignment, mutation calling, and quality control for viral genomes.