Fig. 2: bts-r (the suppressor of bts-2) exhibited reduced low-temperature sensitivity and iron deficiency tolerance.

a bts-r is not sensitive to low temperatures. Col-0, bts-2, and bts-r plants were grown at 22 °C for 4 weeks (upper panel) or grown at 22 °C for 4 weeks then transferred to 4 °C for 7 days (lower panel). Bar = 1 cm. b bts-r did not accumulate H₂O₂ under low temperature. The plants were grown at 22 °C for 4 weeks and transferred to 4 °C for another 2 days, and then the leaves were stained with DAB and trypan blue. Bar = 100 μm. The experiment was repeated at least three times with six individual leaves each time. c bts-r was sensitive to iron deficiency. The mutants were grown on ½ MS medium, which contains 100 μM Fe(II)-EDTA (hereafter as a mock), and the iron-deficient condition was made by applying 300 μM Ferrozine in ½ MS medium to chelate iron (lower panel) for 7 days. d Statistical analysis of root length for the plants in (c). The 7-day-old root length of the plants was measured. Data presented as means ± SD (n = 10 biological replicates) by two-factor ANOVA with Tukey’s HSD test (P ≤ 0.05). e The phenotypes of bts-r plants under iron deficiency. The plants were grown on ½ MS at 22 °C for 7 days and then transferred to ½ MS medium containing 100 μM Fe(II)-EDTA (mock) or 300 μM Ferrozine (iron deficiency) for 5 days, respectively. f Chlorophyll concentration of the plants in (e). Six plants per replicate were collected and used for chlorophyll concentration measurement. Data presented as means ± SD (n = 3 biological replicates) by two-factor ANOVA with Tukey’s HSD test (P ≤ 0.05). g Iron concentration in Col-0, bts-2, and bts-r plants. Fe concentration is on the dry weight basis, and leaves from plants grown in soil under 22 °C for 4 weeks were used for Fe measurement. About twenty plants for per replicate were used. Data presented as means ± SD (n = 6 biological replicates) by one-way ANOVA with Tukey’s HSD test (P ≤ 0.05). Source data are provided as a Source Data file.