Fig. 5: RH24 is genetically epistatic to BTS.

a Chilling sensitivity of Col-0, rh24-1, rh24-2, and RH24-ox plants in darkness. The plants were grown on ½ MS medium, which contains 100 or 300 μM Fe(II)-EDTA. Left panel, 22 °C for 10 d; right panel, 4°C in the dark for 21 d. b Quantification of hypocotyl elongation of plants shown in (a). Hypocotyl elongation of the plants were compared with Col-0 at 22 °C or 4 °C under control conditions (100 μM Fe). The value of Col-0 was set as 100%. Data presented as mean ± SD (n = 5 biological replicates) by two-factor ANOVA with Tukey’s HSD test (P ≤ 0.05). c The phenotype of Col-0, rh24-1, rh24-2, and RH24-ox plants under iron and chilling stresses. Plants were grown on ½ MS medium at 22 °C for 7 days and then transferred to ½ MS medium which contains 100 or 300 μM Fe(II)-EDTA at 22 °C for 1 week (left panel) and then treated with 4 °C for another 2 weeks (right panel). d Chlorophyll concentration of the plants in (c). Six plants of replicate were collected and used for chlorophyll concentration measurement. Data presented as means ± SD (n = 3 biological replicates) by two-factor ANOVA with Tukey’s HSD test (P ≤ 0.05). e Iron concentration in Col-0, bts-2, rh24-1, rh24-2, and rh24-1/bts-2 plants. The plants were grown under 22°C for 4 weeks, and the leaves were used for Fe measurement. About twenty plants were used for each replicate. Data presented as means ± SD (n = 6 biological replicates) by one-way ANOVA with Tukey’s HSD test (P ≤ 0.05). f The phenotype of rh24-1/bts-2 mutants under iron deficiency. The mutants were grown on ½ MS for 5 days and then transferred to ½ MS medium, which contains 100 μM Fe(II)-EDTA (mock) or 300 μM Ferrozine (iron deficiency) in the medium (right panel) for 7 days at 22 °C. g Statistical analysis of root length for the plants in (f). The root length of 12-day-old plants was measured. Data presented as means ± SD (n = 4 biological replicates) by two-factor ANOVA with Tukey’s HSD test (P ≤ 0.05). Source data are provided as a Source Data file.