Fig. 4: Frequency and phenotype of ex vivo MPXV-specific T cell response in convalescent and vaccinated donors. | Nature Communications

Fig. 4: Frequency and phenotype of ex vivo MPXV-specific T cell response in convalescent and vaccinated donors.

From: T cell memory response to MPXV infection exhibits greater effector function and migratory potential compared to MVA-BN vaccination

Fig. 4

A Study design for MPXV-convalescent and vaccinated individuals (created in BioRender. Antoun, E. (2025) https://BioRender.com/a25q267). B Summary of VACV-induced memory T cell response in mpox convalescent (N = 13) and in individuals 28 days after 1 vaccination (N = 20) and 28 days after 2 vaccinations (N = 16). C Representative plot of tetramer+CD8+ and overall CD8+ T cells. D Comparison of frequency of HLA-A*02:01 ILD-specific Tetramer+ T cells between convalescent (N = 5) and vaccinated donors (N = 6). Boxplots represent the 25th and 75th percentiles with the median marked with whiskers at ±1.5*IQR. E Percentage of different memory and differentiation phenotype marker expression on CD8+tetramer+ T cells from convalescent participants (red, N = 5) and samples 28 days after 2 vaccinations (blue, N = 6). F Expression of memory and differentiation markers on CD45RA+CCR7-CD8+tetramer+ T cells from convalescent participants (red, N = 5) and samples 28 days after dose 2 of vaccine (blue, N = 6). Data are presented as median±IQR. The Mann–Whitney U-test was used and two-tailed P values were calculated. SFU spot forming units. Source data are provided as a Source Data file.

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