Fig. 4: VSV-G boosts endosomal escape following receptor-mediated endocytosis of engineered EVs into recipient cells. | Nature Communications

Fig. 4: VSV-G boosts endosomal escape following receptor-mediated endocytosis of engineered EVs into recipient cells.

From: Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors

Fig. 4

a, b Properties of the two VSV-G mutants: VSV-G P127D loses the capacity to mediate fusion between the EV-and endosomal membranes and VSV-G K47Q has a reduced capacity to bind to LDLR on the cell surface. c Confocal immunofluorescence demonstrating the subcellular distribution of mNG in the presence or absence of wild type VSV-G engineered EVs in Huh7 cells. Scale bar, 20 µm, representative images. d Subcellular distribution of mNG in different groups after adding the indicated engineered EVs determined by confocal immunofluorescence. Scale bar, 20 µm, representative images. e WB evaluation of protein levels of Cre and VSV-G in HeLa-TL reporter cells after addition of engineered EVs with wild type, P127D or K47Q VSV-G in 24-well plates. (f–h) Percentage of GFP positive HeLa-TL, T47D-TL and B16F10-TL cells after adding wild type, P127D or K47Q VSV-G plus CD63-Intein-Cre EVs, as evaluated by flow cytometry. Two-way ANOVA (Tukey) multiple comparisons test was used for analysis of (f–h). Experiments were done with 3 biological replicates and data are shown as mean ± SD. a, b Created in BioRender.com, Zheng, W. (2025) https://BioRender.com/n76v160 and Zheng, W. (2025) https://BioRender.com/n89p785 respectively. Exact statistical analysis was reported in the Source data and Source data are provided as a Source Data file.

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