Fig. 2: Overview of the interactions of tigecycline with the human mitoribosome.
From: T cell toxicity induced by tigecycline binding to the mitochondrial ribosome
A Chemical structure of tigecycline. B Overview of the three tigecycline (purple) binding sites in complex with the human mitoribosome comprising the mtSSU (yellow), mtLSU (blue), mRNA (red), and P-site tRNA (green). The mL45 (magenta) is depicted with its N-terminal extension occupying the exit tunnel. C mtSSU site: tigecycline overlaps with the A-tRNA binding site and is stabilized through multiple interactions. The residues from 12S rRNA which makes direct contact with tigecycline are shown. D mtLSU site-1: a novel tigecycline binding site located at helix 71 of the 16S rRNA, close to the acceptor stem of P-tRNA. E mtLSU site-2: tigecycline is positioned in the PTC where it stacks against the neighboring nucleotides and makes potential contact with mL45 N-terminus. Carved EM density is shown for tigecycline.
