Fig. 3: The three LIT modalities enable applications requiring high sensitivity, rapid analysis, and minimal sample volume.
From: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array
Schematic illustrations of key workflow steps of Ultrasensitive LIT (a) and rationale behind tyramide signal amplification technology (b). Figure was created with MedPeer (medpeer.cn). c LODs of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF measured by four LIT modalities: Ultrasensitive LIT, Speedy LIT, Microvolume LIT and Standard LIT. d-f. Dose dependent median fluorescence intensities of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and each of the other LIT modalities: Ultrasensitive LIT, Speedy LIT, and Microvolume LIT (red or green). Data presented as mean ± SD from three replicates. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.
