Fig. 5: Performance comparison of LIT, Luminex, and ELISA in detecting targets in serum samples.
From: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array
a Dose-dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at various concentrations compared between Standard LIT assays with (red) and without (black) added serum. b Comparison of LODs for IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF with (pink) and without (blue) serum. c Spike recoveries for cytokines at high, medium, and low analyte concentrations. Linear regression plots comparing serum concentrations of IL-6 (d) and IL-8 (e) as determined by Luminex and LIT. Linear regression plots for IL-6 (f) and IL-8 (g) serum concentrations, determined by ELISA and LIT. Linear regression plots for IL-6 (h) and IL-8 (i) serum concentrations, determined by chemiluminescence and LIT. Data are presented as mean ± SD; n = 3 repeated tests. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.
