Table 1 Comparative performance of four LIT modes, Luminex, and ELISA
From: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array
Standard LIT | Speedy LIT | Microvolume LIT | Ultrasensitive LIT | ELISA | Luminex | |
|---|---|---|---|---|---|---|
Sensitivity | pg/ml | pg/ml | pg/ml | fg/ml | pg/ml | pg/ml |
Dynamic range | 4 logs | 4 logs | 4 logs | 3 logs | 3 logs | 4 logs |
Throughput | Multiplex | Multiplex | Multiplex | Multiplex | Single-plex | Multiplex |
Incubation time | 1 h | 15 min | 1 h | 1.5 h | 5 h | 3.5 h |
Reaction volume | 50 μl | 50 μl | 10 μl | 50 μl | 100 μl | 50–100 μl |
