Fig. 5: Selective EGFR deletion in neutrophils attenuated development of kidney fibrosis after ischemia injury. | Nature Communications

Fig. 5: Selective EGFR deletion in neutrophils attenuated development of kidney fibrosis after ischemia injury.

From: Myeloid EGFR deficiency accelerates recovery from AKI via macrophage efferocytosis and neutrophil apoptosis

Fig. 5

A Schematic of experimental protocol. B–D, G NeutEGFR−/− mouse kidney had less injury, indicated by: B lower mRNA levels of Havcr1/Kim-1 (n = 6 and 8) and less fibrosis, indicated by: C lower protein (n = 5) and D mRNA levels (n = 6 and 8) of profibrotic and fibrotic components and G decreased quantitative Picrosirius red staining (n = 6). NeutEGFR−/− mice had less kidney immune cell infiltration, indicated by: E lower mRNA levels of Emr1/F4/80, Cd68 and Ly6g/Gr1 (n = 6 and 8), F macrophage and neutrophil numbers (n = 6 and 7), and G quantitative F4/80 and Gr-1 staining (n = 6). Scale bar = 50 μm. Data are means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using 2 tailed Student’s t test.

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