Fig. 3: Astrocytes have normal basal glucose levels in GLUT1 cKO mice.
From: Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to stroke
a Experimental timeline: Intracortical injection of AAVs encoding glucose sensor FLII12 Pglu700μΔ6 (FLIIP) and DIO-TdTomato into ~8-week-old GLUT1fl/fl;GLASTCreERT2/+ (cKO) and GLUT1+/+;GLASTCreERT2/+ (Ctrl) mice, followed by tamoxifen treatment 7 days later. Acute cortical slice two-photon (2 P) imaging was performed ~60 days post-tamoxifen treatment. b Example 2P images of cortical astrocytes co-expressing FLIIP and TdTomato (marking cells targeted for Cre recombination) selected for glucose imaging (observed in 5 Ctrl and 6 cKO mice). Scale bar: 10 µm. c Representative color-coded glucose sensor ratio images from Ctrl and cKO astrocytes in ACSF with 5 mM glucose ([Glc]O) and after removal of extracellular glucose (0 mM [Glc]O). Warm and cold colors indicate high and low glucose levels, respectively. d Quantification of raw sensor ratios from astrocytes under conditions in (c). At 5 mM [Glc]O and 0 mM [Glc]O, glucose sensor ratios were comparable between Ctrl (n = 63 cells from 9 slices, 5 mice) and cKO (n = 101 cells from 12 slices, 6 mice) (p = 0.536 at 5 mM [Glc]O; p = 0.690 at 0 mM [Glc]O, two-sided linear mixed model). e Normalized glucose sensor ratios (5 mM [Glc]O ratios normalized to the average minimum at 0 mM [Glc]O) show comparable basal glucose levels between genotypes (p = 0.831, two-sided linear mixed model). Box plots show the median (center line), quartiles (box bounds), mean (+) and min-to-max (whiskers). Source data are provided as a Source Data file.
