Fig. 7: ICP expression and IFN-γ signaling in BC.
From: HIV status alters immune cell infiltration and activation profile in women with breast cancer
A Representative pictures of panCK+ cancer cells (gray), HLA-I HC (yellow), pStat1 (red), CTLA4 (orange), and LAG3 (green). The scale bar depicts 50 µm. B–D The expression of ICP molecules CTLA4, PD-1 and LAG3 were tested on different T cell subpopulations. The mean is given as a dashed line and the standard deviation is given as a dot line. Categories were compared using the Mann–Whitney U test and p-values are given as numbers if p < 0.05. E–I The expression of pStat1, ISG15, HLA-I HC, HLA-G and TAP1. The mean is given as a dashed line and the standard deviation is given as a dot line. Categories were compared using the Mann–Whitney U test and p-values are given as numbers if p < 0.05. J Volcano plot depiction of differentially expressed genes in WLWH versus HIV negative BC (GSE149156) for all genes and K immune related genes. Significantly upregulated genes are marked in red, significantly downregulated genes in blue. L Bar plot depiction of GSEA (MSigDB_Hallmark_2020) of WLWH versus HIV negative. The NES is given as the value of the bars and the FDR is depicted in different colors. Gene sets with a significant up-/down-regulation are highlighted with bold letters. Abbreviations: CXCR4 CXC motif chemokine receptor 4, CTLA4 cytotoxic T-lymphocyte-associated protein 4, GSEA gene set enrichment analysis, HLA human leucocyte antigen, ISG15 Interferon-stimulated gene 15, LAG3 lymphocyte-activation gene 3, panCK pan-cytokeratin, PD-1 programmed cell death protein 1, TAP transporter of antigen processing, WLWH women living with HIV.
