Fig. 4: Pwwp3a−/− mice exhibit elevated induction of antiviral cytokines and enhanced resistance against RNA viral infection.
Survival rate of sex- and age-matched Pwwp3a+/+ (blue) and Pwwp3a−/− (orange) mice (n = 6) which were intravenously (i.v.) infected with vesicular stomatitis virus (VSV, 2 × 107 PFU per mouse, a) or herpes simplex virus type 1(HSV-1, 2 × 107 PFU per mouse, c), as well as intranasal inhalation infected with influenza virus A PR8 (106 EID50, b). The survival of mice was recorded every 12 h. The graphs of survival rate were shown by Kaplan-Meier method and analyzed with log-rank test. The ELISA analysis of the levels of indicated cytokines in sera from Pwwp3a+/+ (blue) and Pwwp3a−/− (orange) mice which were i.v. infected with VSV (2 × 107 PFU per mouse, n = 4, d) or HSV-1 (2 × 107 PFU per mouse, n = 6, f), as well as intranasal inhalation infected with influenza virus A PR8 (106 EID50, n = 6, e) at the indicated time points. RT-qPCR analysis of the mRNA abundance of indicated genes in lungs or brains from Pwwp3a+/+ (blue) and Pwwp3a−/− (orange) mice which were i.v. infected with VSV (2 × 107 PFU per mouse, n = 3, g) or HSV-1 (2 × 107 PFU per mouse, n = 3, i), as well as intranasal inhalation infected with influenza virus A PR8 (106 EID50, n = 3, h) for 3 days. Graphs shown as mean ± SD, p values are from log-rank analysis (a–c) or two-tailed, unpaired t-test (d–i). At least three independent experiments were performed with similar results. Source data are provided as a Source Data file.
