Table 1 Benchmarking results of de novo transcriptome assembly for plant RiPP precursor discovery

From: Large-scale transcriptome mining enables macrocyclic diversification and improved bioactivity of the stephanotic acid scaffold

De novo assembler

Total time [min]

Total memory usage [GB]

Total detected cores (138 total cores)

Detected unique correct cores (65 total unique cores)

Detected repetitive cores (120 total repetitive cores)

Similarity [sum %] (total similarity score: 3600)

Identity [sum %] (total identity score: 3600)

MEGAHIT (v1.2.9)

1371 ± 66

210 ± 0

67 ± 0

38 ± 0

51 ± 0

2741 ± 0

2738 ± 0

SPAdes (v3.15.5)

1627 ± 42

241 ± 3

93 ± 0

48 ± 0

74 ± 0

3012 ± 0

2981 ± 0

Trinity (v2.15.1)

21833 ± 558

813 ± 23

64 ± 0

37 ± 0

44 ± 1

2618 ± 10

2597 ± 7

  1. 27 RNA-seq datasets were trimmed and assembled which include 36 precursor genes with 138 core peptides and 65 unique core peptides. Each RNA-seq dataset was assembled in triplicate (n = 3). Displayed values are means with one standard deviation shown as an error. Source data are provided as a Source Data file.