Fig. 3: Elucidating HAN2-mediated chilling tolerance via auxin signalling.
From: Suppressing an auxin efflux transporter enhances rice adaptation to temperate habitats
a Chilling tolerance phenotype of 93-11 and NIL-HAN2NPB plants. b Survival rates of 93-11 and NIL-HAN2NPB plants with and without auxin recovered after chilling treatment. Data are shown as the means ± SD (n = 4 biologically replicates, with 16 seedlings per biological replicate). c The chilling tolerance phenotype of WT and han2 plants. d Survival rates of WT and han2 plants with and without auxin recovered after chilling treatment. Data are shown as the means ± SD (n = 4 biologically replicates, with 16 seedlings per biological replicate). e The OsMAP1/OsMPK3 expression in both 93-11 and NIL-HAN2NPB before and after chilling treatment. Data are shown as the means ± SD (n = 3 biologically replicates, with 3 seedlings per biological replicate). f The OsMAP1/OsMPK3 expression in both WT and han2 before and after chilling treatment. Data are shown as the means ± SD (n = 4 biologically replicates, with 3 seedlings per biological replicate). g OsARF24 negatively regulates the transcriptional activity of OsMAP1/OsMPK3 promoter in rice protoplasts. Data are shown as the means ± SD (n = 3 biologically replicates). h Y1H assays demonstrate that OsARF24 interacts with ARF-motif elements. i EMSA confirms that OsARF24 binds to the ARF-motifs of OsMAP1/OsMPK3 promoter. j ChIP-qPCR showing that OsARF24 binds to the OsMAP1/OsMPK3 promoter in vivo. P1-P6, represent DNA fragments from different positions of the OsMAP1/OsMPK3 promoter. Immunoprecipitation was performed with anti-Flag antibody (IP), IgG was used as a negative control (CK). Actin used as the internal standard. Data are shown as the means ± SD (n = 4 biologically replicates). k OsMAP1/OsMPK3 expression in WT and OsARF24-OE lines. Data are shown as the means ± SD (n = 5 biologically replicates, with 3 seedlings per biological replicate). l Distribution of OsARF24 free and compound proteins in OsARF24-Flag/ZH11 overexpressing plants with and without auxin. A representative experiment from at least two independent experiments is shown. Significance is evaluated by the two-tailed Student’s t-test, and P-values are indicated. Source data are provided as a Source Data file.
