Fig. 5: Reconstruction of the in vitro BBB model.
From: Fusogenic lipid nanoparticles for rapid delivery of large therapeutic molecules to exosomes
a A scheme showing a 3D BBB model with three-phase guide channels. b Entire image of the microfluidic device and the process of creating an in vitro 3D BBB model and permeability test. c Confocal image showing cells cultured in the 3D BBB model. d In the 3D BBB model, human brain microvascular endothelial cells (HBMEC), human astrocytes (HA), and human brain vesicular pericytes (HBVP) are labeled with CD31(green), GFAP(red), and αSMA(orange), respectively. e Visualization of tight junction proteins in the endothelial cell layer, confirming the integrity and maturity of the endothelial barrier: CD31 (green), ZO-1 (yellow), VE-cadherin (purple), and Claudin-5 (cyan). a Created in BioRender. Song, J. (2025) https://BioRender.com/o9v2cps.
